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02595nam a22003135i 4500 |
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20210803161840.0 |
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cr nn 008mamaa |
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130220s2013 xxu| s |||| 0|eng d |
| 020 |
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|a 9781627032933
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| 024 |
7 |
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|a 10.1007/978-1-62703-293-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Enzyme Engineering :
|b Methods and Protocols /
|c edited by James C. Samuelson.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XI, 252 p. 52 illus., 19 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 978
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| 505 |
0 |
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|a A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivo -- Determining Enzyme Kinetics via Isothermal Titration Calorimetry -- GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria -- Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage Properties -- TAL Effector Nuclease (TALEN) Engineering -- In vitro Evolution of Enzymes -- Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivo -- Reconstructing Evolutionary Adaptive Paths for Protein Engineering -- Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria -- FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants -- Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes -- Gene Synthesis by Assembly of Deoxyuridine Containing Oligonucleotides -- Protein Engineering: Single or Multiple Site-Directed Mutagenesis -- Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination -- Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments -- Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis -- Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter -- In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre Plates.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Enzymology.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Enzymology.
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| 700 |
1 |
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|a Samuelson, James C.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|