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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592594306
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| 024 |
7 |
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|a 10.1385/1592594301
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a G Protein Signaling :
|b Methods and Protocols /
|c edited by Alan V. Smrcka.
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| 250 |
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|a 1st ed. 2004.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XII, 250 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 237
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| 505 |
0 |
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|a Purification of G Protein Subunits and G Protein Effectors -- Purification of Recombinant G Protein ? Subunits from Escherichia coli -- Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged ? and ?? Subunits -- Expression and Purification of Soluble Adenylyl Cyclase from Escherichia coli -- Purification of Phospholipase C ? and Phospholipase C ? from Sf9 Cells -- Assays for G Protein—Dependent Effector Activation In Vitro -- Assay for G Protein-Dependent Activation of Phospholipase C ? Using Purified Protein Components -- Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cells -- Assays For G Protein-Coupled Receptor-Mediated and G Protein Subunit-Mediated Effector Activation in Intact Cells -- Measurement of G Protein-Coupled Receptor-Stimulated Phospholipase D Activity in Intact Cells -- Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cells -- Receptor Mechanisms, Activity, and Localization -- Intensive Mutational Analysis of G Protein-Coupled Receptors in Yeast -- Green Fluorescent Protein-Tagged ?-Arrestin Translocation as a Measure of G Protein-Coupled Receptor Activation -- Detection of G Protein-Coupled Receptors by Immunofluorescence Microscopy -- Assay of G Protein-Coupled Receptor Activation of G Proteins in Native Cell Membranes Using [35S]GTP?S Binding -- Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay -- Disruption of Endogenous G Protein-Signaling Systems to Understand the Role of G Protein Subunits in Intact Systems -- Selective Inhibition of G Protein-Mediated Pathways Using RGS Domains -- Ribozymes as Tools for Suppression of G Protein ? Subunits -- In Vivo Adenoviral-Mediated Gene Transfer of the ?ARKct to Study the Role of G?? in Arterial Restenosis -- Posttranslational Modifications of Regulators of G Protein Signaling -- Analysis of RGS Protein Palmitoylation -- Methods for Measuring RGS Protein Phosphorylation by G Protein-Regulated Kinases -- Use of Gfp-Tagged G Proteins and Effectors in Intact Cells -- The Use of Green Fluorescent Proteins to View Association Between Phospholipase C? and G Protein Subunits in Cells -- Cellular Localization of GFP-Tagged ? Subunits.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Smrcka, Alan V.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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