G Protein Signaling : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
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| Edición: | 1st ed. 2004. |
| Colección: | Methods in Molecular Biology,
237 |
| Materias: |
Tabla de Contenidos:
- Purification of G Protein Subunits and G Protein Effectors
- Purification of Recombinant G Protein ? Subunits from Escherichia coli
- Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged ? and ?? Subunits
- Expression and Purification of Soluble Adenylyl Cyclase from Escherichia coli
- Purification of Phospholipase C ? and Phospholipase C ? from Sf9 Cells
- Assays for G Protein—Dependent Effector Activation In Vitro
- Assay for G Protein-Dependent Activation of Phospholipase C ? Using Purified Protein Components
- Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cells
- Assays For G Protein-Coupled Receptor-Mediated and G Protein Subunit-Mediated Effector Activation in Intact Cells
- Measurement of G Protein-Coupled Receptor-Stimulated Phospholipase D Activity in Intact Cells
- Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cells
- Receptor Mechanisms, Activity, and Localization
- Intensive Mutational Analysis of G Protein-Coupled Receptors in Yeast
- Green Fluorescent Protein-Tagged ?-Arrestin Translocation as a Measure of G Protein-Coupled Receptor Activation
- Detection of G Protein-Coupled Receptors by Immunofluorescence Microscopy
- Assay of G Protein-Coupled Receptor Activation of G Proteins in Native Cell Membranes Using [35S]GTP?S Binding
- Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay
- Disruption of Endogenous G Protein-Signaling Systems to Understand the Role of G Protein Subunits in Intact Systems
- Selective Inhibition of G Protein-Mediated Pathways Using RGS Domains
- Ribozymes as Tools for Suppression of G Protein ? Subunits
- In Vivo Adenoviral-Mediated Gene Transfer of the ?ARKct to Study the Role of G?? in Arterial Restenosis
- Posttranslational Modifications of Regulators of G Protein Signaling
- Analysis of RGS Protein Palmitoylation
- Methods for Measuring RGS Protein Phosphorylation by G Protein-Regulated Kinases
- Use of Gfp-Tagged G Proteins and Effectors in Intact Cells
- The Use of Green Fluorescent Proteins to View Association Between Phospholipase C? and G Protein Subunits in Cells
- Cellular Localization of GFP-Tagged ? Subunits.