G Protein Signaling : Methods and Protocols /

G Protein Signaling : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Smrcka, Alan V. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edition:1st ed. 2004.
Series:Methods in Molecular Biology, 237
Subjects:
Cell biology.
Cell Biology.
Table of Contents:
  • Purification of G Protein Subunits and G Protein Effectors
  • Purification of Recombinant G Protein ? Subunits from Escherichia coli
  • Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged ? and ?? Subunits
  • Expression and Purification of Soluble Adenylyl Cyclase from Escherichia coli
  • Purification of Phospholipase C ? and Phospholipase C ? from Sf9 Cells
  • Assays for G Protein-Dependent Effector Activation In Vitro
  • Assay for G Protein-Dependent Activation of Phospholipase C ? Using Purified Protein Components
  • Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cells
  • Assays For G Protein-Coupled Receptor-Mediated and G Protein Subunit-Mediated Effector Activation in Intact Cells
  • Measurement of G Protein-Coupled Receptor-Stimulated Phospholipase D Activity in Intact Cells
  • Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cells
  • Receptor Mechanisms, Activity, and Localization
  • Intensive Mutational Analysis of G Protein-Coupled Receptors in Yeast
  • Green Fluorescent Protein-Tagged ?-Arrestin Translocation as a Measure of G Protein-Coupled Receptor Activation
  • Detection of G Protein-Coupled Receptors by Immunofluorescence Microscopy
  • Assay of G Protein-Coupled Receptor Activation of G Proteins in Native Cell Membranes Using [35S]GTP?S Binding
  • Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay
  • Disruption of Endogenous G Protein-Signaling Systems to Understand the Role of G Protein Subunits in Intact Systems
  • Selective Inhibition of G Protein-Mediated Pathways Using RGS Domains
  • Ribozymes as Tools for Suppression of G Protein ? Subunits
  • In Vivo Adenoviral-Mediated Gene Transfer of the ?ARKct to Study the Role of G?? in Arterial Restenosis
  • Posttranslational Modifications of Regulators of G Protein Signaling
  • Analysis of RGS Protein Palmitoylation
  • Methods for Measuring RGS Protein Phosphorylation by G Protein-Regulated Kinases
  • Use of Gfp-Tagged G Proteins and Effectors in Intact Cells
  • The Use of Green Fluorescent Proteins to View Association Between Phospholipase C? and G Protein Subunits in Cells
  • Cellular Localization of GFP-Tagged ? Subunits.

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