Detection of Blotted Proteins : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edición: | 1st ed. 2015. |
| Colección: | Methods in Molecular Biology,
1314 |
| Materias: |
Tabla de Contenidos:
- Western Blotting: Origin and Ascent of the Species
- Methods to Concentrate Proteins for Protein Isolation, Proteomic and Peptidomic Evaluation
- Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose
- Detection of Blotted Proteins: Not all Blockers are Created Equal
- Protein Stains to Detect Antigen on Membranes
- Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting
- Rapid, Antibody-free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation
- Use of Non-Radioactive Detection Method for North- and South-Western Blot
- Immunoblotting using Radiolabeled Reagents for Detection
- Immunoblotting of Antigens: Whole, Strip and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique
- Detection of Protein Carbonyls by Means of Biotin Hydrazide –Streptavidin Affinity Methods
- Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel
- Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays
- Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
- Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL-plex Immunoblotting in a Standard Proteomics Workflow
- Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot blots and Immunohistochemistry
- Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane
- Sequential use of Immunoblots for Characterization of Autoantibody Specificities
- Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western Blotting
- Application of Intermittent Microwave Irradiation to Western Blot Analysis
- Visualization of Unstained Protein Bands on PVDF
- Multiplexed Fluorescent Immunodetection Using Low Auto-Fluorescence Immobilon®-FL Membrane
- Cold Microwave-Enabled Protein Detection and Quantification
- TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics
- Analysis of Electroblotted Proteins by Mass Spectrometry
- On Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions
- Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore
- Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane
- Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta
- Non-stripping “Rainbow” and Multiple Antigen Detection (MAD) Western Blotting. - Supported Molecular Matrix Electrophoresis
- Parafilm-M®, an Available Cost Effective Alternative for Immuno-blot Pouches
- Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes
- Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva
- A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMB
- Other Notable Methods of Membrane Protein Detection: A Brief Review
- Nitrocellulose Membrane: The New Canvas
- Invisible Ink Marking in ECL Membrane Assays. .