Glycomics : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Packer, Nicolle H. (Editor ), Karlsson, Niclas G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 534
Materias:
Tabla de Contenidos:
  • Glycan Analysis
  • Analysis of N- and O-Linked Glycans from Glycoproteins Using MALDI-TOF Mass Spectrometry
  • Infrared Multiphoton Dissociation Mass Spectrometry for Structural Elucidation of Oligosaccharides
  • Mass Spectrometry of N-Linked Glycans
  • Solid-Phase Permethylation for Glycomic Analysis
  • Method for Investigation of Oligosaccharides Using Phenylhydrazine Derivatization
  • Two-Dimensional HPLC Separation with Reverse-Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools After Labeling with 2-Aminobenzamide
  • Capillary Lectin-Affinity Electrophoresis for Glycan Analysis
  • Analysis of Methylated O-Glycan Alditols by Reversed-Phase NanoLC Coupled CAD-ESI Mass Spectrometry
  • High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling
  • Collision-Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans
  • The Structural Elucidation of Glycosaminoglycans
  • Labelling Heparan Sulphate Saccharides with Chromophore, Fluorescence and Mass Tags for HPLC and MS Separations
  • Small-Scale Enzymatic Digestion of Glycoproteins and Proteoglycans for Analysis of Oligosaccharides by LC-MS and FACE Gel Electrophoresis
  • Glycopeptides
  • Enrichment Strategies for Glycopeptides
  • Introductory Glycosylation Analysis Using SDS-PAGE and Peptide Mass Fingerprinting
  • Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF
  • N-Glycosylation Site Analysis of Human Platelet Proteins by Hydrazide Affinity Capturing and LC-MS/MS
  • LC/MS n for Glycoprotein Analysis: N-Linked Glycosylation Analysis and Peptide Sequencing of Glycopeptides
  • O-GlcNAcomics
  • Detecting the “O-GlcNAcome”; Detection, Purification, and Analysis of O-GlcNAc Modified Proteins
  • Glycobioinformatic Tools
  • Preparation of a Glycan Library Using a Variety of Glycosyltrasferases
  • Data Mining the PDB for Glyco-Related Data
  • Carbohydrate-Protein Interactions
  • Saccharide Microarrays for High-Throughput Interrogation of Glycan-Protein Binding Interactions
  • Glycosaminoglycan Characterization Methodologies: Probing Biomolecular Interactions
  • Development and Characterization of Antibodies to Carbohydrate Antigens
  • Inhibition of Glycosyltransferase Activities as the Basis for Drug Development
  • Saturation Transfer Difference NMR Spectroscopy as a Technique to Investigate Protein-Carbohydrate Interactions in Solution.