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110506s2011 xxu| s |||| 0|eng d |
| 020 |
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|a 9781617791208
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| 024 |
7 |
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|a 10.1007/978-1-61779-120-8
|2 doi
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| 040 |
|
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Cystic Fibrosis :
|b Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis /
|c edited by Margarida D. Amaral, Karl Kunzelmann.
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| 250 |
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|a 1st ed. 2011.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2011.
|
| 300 |
|
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|a XIV, 384 p. :
|b online resource.
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 742
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| 505 |
0 |
|
|a Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology -- Imaging CFTR Protein Localization in Cultured Cells and Tissues -- CFTR Regulation of Epithelial Sodium Channel -- Methods for Evaluating Inflammation in Cystic Fibrosis -- Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures -- Measurement of Fluid Secretion from Intact Airway Submucosal Glands -- Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia -- Identification and Quantification of Mucin Expression -- Methods to Classify Bacterial Pathogens in Cystic Fibrosis -- Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells -- Introduction to Part II: Omics in the Biology of Cystic Fibrosis -- Microarray mRNA Expression Profiling to Study Cystic Fibrosis -- Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture) -- Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis -- Functional Genomics Assays to Study CFTR Traffic and ENaC Function -- New Lipidomic Approaches in Cystic Fibrosis -- Introduction to Part III: Resources for CFTR Research -- Primary Epithelial Cell Models for Cystic Fibrosis Research -- Comparative Biology of Cystic Fibrosis Animal Models -- CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction -- Evaluation of the Disease Liability of CFTR Variants.
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| 650 |
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0 |
|a Molecular biology.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
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0 |
|a Laboratory medicine.
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| 650 |
1 |
4 |
|a Molecular Medicine.
|
| 650 |
2 |
4 |
|a Human Genetics.
|
| 650 |
2 |
4 |
|a Laboratory Medicine.
|
| 700 |
1 |
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|a Amaral, Margarida D.
|e editor.
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| 700 |
1 |
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|a Kunzelmann, Karl.
|e editor.
|
| 710 |
2 |
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|a SpringerLink (Online service)
|
| 773 |
0 |
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|t Springer eBooks
|