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20210420135112.0 |
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110519s2011 xxu| s |||| 0|eng d |
| 020 |
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|a 9781617791178
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| 024 |
7 |
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|a 10.1007/978-1-61779-117-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Cystic Fibrosis :
|b Diagnosis and Protocols, Volume I: Approaches to Study and Correct CFTR Defects /
|c edited by Margarida D. Amaral, Karl Kunzelmann.
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| 250 |
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|a 1st ed. 2011.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2011.
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| 300 |
|
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|a XVI, 528 p. 76 illus., 4 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 741
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| 505 |
0 |
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|a Introduction to Part I: The Relevance of CF Diagnostic Tools for Measuring Restoration of CFTR Function after Therapeutic Interventions in Human Clinical Trials -- High-Throughput Screening of Libraries of Compounds to Identify CFTR Modulators -- Repair of CFTR Folding Defects with Correctors that Function as Pharmacological Chaperones -- Use of Primary Cultures of Human Bronchial Epithelial Cells Isolated from Cystic Fibrosis Patients for the Pre-Clinical Testing of CFTR Modulators -- Design of Gene Therapy Trials in CF Patients -- Nasal Potential Difference Measurements to Assess CFTR Ion Channel Activity -- Measurement of Ion Transport Function in Rectal Biopsies -- Introduction to Part II: RNA Methods to Approach CFTR Expression -- Quantification of CFTR Transcripts -- Nonsense-Mediated mRNA Decay and Cystic Fibrosis -- Approaches to Study CFTR Pre-mRNA Splicing Defects -- Impact of MicroRNA in Normal and Pathological Respiratory Epithelia -- Genomic Approaches to Studying CFTR Transcriptional Regulation -- Introduction to Part III: Biochemical Methods to Study CFTR Protein -- Analysis of CFTR Folding and Degradation in Transiently Transfected Cells -- In Vitro Methods for CFTR Biogenesis -- Analysis of CFTR Interactome in the Macromolecular Complexes -- Methods to Monitor Cell Surface Expression and Endocytic Trafficking of CFTR in Polarized Epithelial Cells -- Segmental and Subcellular Distribution of CFTR in the Kidney -- Endocytic Sorting of CFTR Variants Monitored by Single Cell Fluorescence Ratio Image Analysis (FRIA) in Living Cells -- Introduction to Part IV: Biophysical Methods to Approach CFTR Structure -- CFTR Three-Dimensional Structure -- Molecular Modeling Tools and Approaches for CFTR and Cystic Fibrosis -- Biochemical and Biophysical Approaches to Probe CFTR Structure -- NMR Spectroscopy to Study the Dynamics and Interactions of CFTR -- Introduction to Part V: Assessment of CFTR Function -- Application of High-Resolution Single-Channel Recording to Functional Studies of Cystic Fibrosis Mutants -- Electrophysiological, Biochemical, and Bioinformatic Methods for Studying CFTR Channel Gating and Its Regulation -- CFTR Regulation by Phosphorylation -- How to Measure CFTR-Dependent Bicarbonate Transport: From Single Channels to the Intact Epithelium.
|
| 650 |
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0 |
|a Molecular biology.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
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0 |
|a Laboratory medicine.
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| 650 |
1 |
4 |
|a Molecular Medicine.
|
| 650 |
2 |
4 |
|a Human Genetics.
|
| 650 |
2 |
4 |
|a Laboratory Medicine.
|
| 700 |
1 |
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|a Amaral, Margarida D.
|e editor.
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| 700 |
1 |
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|a Kunzelmann, Karl.
|e editor.
|
| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|