Cystic Fibrosis : Diagnosis and Protocols, Volume I: Approaches to Study and Correct CFTR Defects /
| Corporate Author: | |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edition: | 1st ed. 2011. |
| Series: | Methods in Molecular Biology,
741 |
| Subjects: |
Table of Contents:
- Introduction to Part I: The Relevance of CF Diagnostic Tools for Measuring Restoration of CFTR Function after Therapeutic Interventions in Human Clinical Trials
- High-Throughput Screening of Libraries of Compounds to Identify CFTR Modulators
- Repair of CFTR Folding Defects with Correctors that Function as Pharmacological Chaperones
- Use of Primary Cultures of Human Bronchial Epithelial Cells Isolated from Cystic Fibrosis Patients for the Pre-Clinical Testing of CFTR Modulators
- Design of Gene Therapy Trials in CF Patients
- Nasal Potential Difference Measurements to Assess CFTR Ion Channel Activity
- Measurement of Ion Transport Function in Rectal Biopsies
- Introduction to Part II: RNA Methods to Approach CFTR Expression
- Quantification of CFTR Transcripts
- Nonsense-Mediated mRNA Decay and Cystic Fibrosis
- Approaches to Study CFTR Pre-mRNA Splicing Defects
- Impact of MicroRNA in Normal and Pathological Respiratory Epithelia
- Genomic Approaches to Studying CFTR Transcriptional Regulation
- Introduction to Part III: Biochemical Methods to Study CFTR Protein
- Analysis of CFTR Folding and Degradation in Transiently Transfected Cells
- In Vitro Methods for CFTR Biogenesis
- Analysis of CFTR Interactome in the Macromolecular Complexes
- Methods to Monitor Cell Surface Expression and Endocytic Trafficking of CFTR in Polarized Epithelial Cells
- Segmental and Subcellular Distribution of CFTR in the Kidney
- Endocytic Sorting of CFTR Variants Monitored by Single Cell Fluorescence Ratio Image Analysis (FRIA) in Living Cells
- Introduction to Part IV: Biophysical Methods to Approach CFTR Structure
- CFTR Three-Dimensional Structure
- Molecular Modeling Tools and Approaches for CFTR and Cystic Fibrosis
- Biochemical and Biophysical Approaches to Probe CFTR Structure
- NMR Spectroscopy to Study the Dynamics and Interactions of CFTR
- Introduction to Part V: Assessment of CFTR Function
- Application of High-Resolution Single-Channel Recording to Functional Studies of Cystic Fibrosis Mutants
- Electrophysiological, Biochemical, and Bioinformatic Methods for Studying CFTR Channel Gating and Its Regulation
- CFTR Regulation by Phosphorylation
- How to Measure CFTR-Dependent Bicarbonate Transport: From Single Channels to the Intact Epithelium.