Gene Knockout Protocols /
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edition: | 2nd ed. 2009. |
| Series: | Methods in Molecular Biology,
530 |
| Subjects: |
Table of Contents:
- Gene Modification in ES Cells
- Overview on Mouse Mutagenesis
- Construction of Gene-Targeting Vectors by Recombineering
- Gene-Trap Vectors and Mutagenesis
- Chromosome Engineering in ES Cells
- Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides
- Generation of shRNA Transgenic Mice
- Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate
- Stem Cell Manipulation
- Gene Targeting in Mouse Embryonic Stem Cells
- Manipulating Mouse Embryonic Stem Cells
- ES Cell Line Establishment
- Generation of Double-Knockout Embryonic Stem Cells
- Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro
- Cloning of ES Cells and Mice by Nuclear Transfer
- Genetically Engineered Mice
- Isolation, Microinjection and Transfer of Mouse Blastocysts
- Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos
- VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos
- Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes
- Inducible Cre Mice
- Creation and Use of a Cre Recombinase Transgenic Database
- Transposon Mutagenesis in Mice
- Lentiviral Transgenesis
- Sperm Cryopreservation and In Vitro Fertilization
- Phenotype Analysis
- Influence of Genetic Background on Genetically Engineered Mouse Phenotypes
- Pathologic Phenotyping of Mutant Mice
- Systemic First-Line Phenotyping.