Extracellular Matrix Protocols : Second Edition /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edición: | 2nd ed. 2009. |
| Colección: | Methods in Molecular Biology,
522 |
| Materias: |
Tabla de Contenidos:
- Molecular Biology
- Retroviral delivery of ECM genes
- Tissue-Specific KO of ECM Proteins
- Recombinant Collagen Trimers from Insect Cells and Yeast
- Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag
- Preparation of recombinant fibronectin fragments for functional and structural studies
- Quantitative Determination of Collagen Cross-links
- Biochemical and biophysical analysis
- ECM Macromolecules: Height-mapping and Nano-mechanics Using Atomic Force Microscopy
- Atomic Force Microscopy Measurements of Intermolecular Binding Forces
- Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy
- Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry (SIMS)
- ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy
- Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates
- Solid Phase Assays for Studying ECM Protein–Protein Interactions
- Cell Adhesion Assays
- Cell biology assays
- ECM Degradation Assays for Analyzing Local Cell Invasion
- Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration
- Fibrin Gel Model for Assessment of Cellular Contractility
- Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis)
- Stromagenesis During Tumorigenesis: Characterization of Tumor-associated Fibroblasts and Stroma-derived 3D Matrices
- Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes
- Organ models
- ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development
- Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice
- Tissue Engineering
- Characterizing ECM Production by Cells Encapsulated in Hydrogels
- Tissue Engineering and Cell-Populated Collagen Matrices.