G Protein-Coupled Receptor Screening Assays : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Prazeres, Duarte Miguel F. (Editor), Martins, Sofia Aires M. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2015.
Edition:1st ed. 2015.
Series:Methods in Molecular Biology, 1272
Subjects:
Table of Contents:
  • G-Protein Coupled Receptors: An Overview of Signaling Mechanisms and Screening Assays
  • Time-Resolved FRET Strategy to Screen GPCR Ligands Library
  • Homogeneous Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs in Budded Baculoviruses: The Case of Cy3B-NDP-a-MSH Binding to MC4 Receptors
  • Construction of Recombinant HEK293 Cell Lines for the Expression of the Neurotensin Receptor NTSR1
  • cAMP Assay for GPCR Ligand Characterization: Application of BacMam Expression System
  • Ca2+ Mobilization Assays in GPCR Drug Discovery
  • Using Constitutive Activity to Define Appropriate High Throughput Screening Assays for Orphan G Protein-Coupled Receptors
  • Monitoring G-Protein Coupled Receptor Activation Using the Protein Fragment Complementation Technique Split TEV
  • Quantifying GPCR Internalization: A Focus on the Kisspeptin Receptor
  • GPCR Oligomerization Analysis by Means of BRET and dFRAP
  • Use of ImageJ to Recover Information from Individual Cells in a G-Protein Coupled Receptor Assay
  • Methods to Immobilize GPCR on the Surface of SPR Sensors
  • Olfactory Receptor Screening Assay Using Nanovesicle-Immobilized Carbon Nanotube Transistor
  • Label-Free Biosensor Assays in GPCR Screening
  • Multi-Dimensional GPCR Profiling and Screening Using Impedance-Based Label-Free and Real-Time Assay
  • Label-Free Functional Selectivity Assays
  • Measurement of Surface-Mediated Ca++ Transients on the Single-Cell Level in a Microfluidic Lab-on-a-chip Environment
  • Cell-Based Assays and Animal Models for GPCR Drug Screening
  • Computer-Aided Design of GPCR Ligands.