Glycosyltransferases : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Brockhausen, Inka. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edition:1st ed. 2013.
Series:Methods in Molecular Biology, 1022
Subjects:
LEADER 04260nam a22003375i 4500
001 000283543
005 20210510140938.0
007 cr nn 008mamaa
008 130614s2013 xxu| s |||| 0|eng d
020 |a 9781627034654 
024 7 |a 10.1007/978-1-62703-465-4  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Glycosyltransferases :  |b Methods and Protocols /  |c edited by Inka Brockhausen. 
250 |a 1st ed. 2013. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2013. 
300 |a XVII, 425 p. 110 illus., 40 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1022 
505 0 |a Glycan Microarray Screening Assay for Glycosyltransferase Specificities -- A Fluorescence-Based Assay for Core 1 3Galactosyltransferase (T-synthase) Activity.- Structural and Biochemical Analysis of a Bacterial Glycosyltransferase -- Study of the Biological Functions of Mucin Type Core 3 O-glycans -- Generation of Anti-Sulfated Glycan Antibodies using Sulfotransferase-deficient Mice -- Fluorescent Microscopy as a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in COG Complex Depleted Mammalian Cells -- A Practical Approach to Reconstruct Evolutionary History of Animal Sialyltransferases and Gain Insights into the Sequence-Function Relationships of Golgi-Glycosyltransferases -- Fluorescent Lectin Staining of Drosophila Embryos and Tissues to Detect the Spatial Distribution of Glycans during Development -- Photoaffinity Labeling of Protein O-Mannosyltransferases of the PMT1/PMT2 Subfamily -- Enzymatic Analysis of the Protein O-glycosyltransferase, Rumi, Acting toward Epidermal Growth Factor-like (EGF) Repeats -- Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling -- Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase, and Assay of its Substrate, UDP-GlcNAc -- In vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases -- In vitro UDP-sugar:Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection by Thin Layer Chromatography -- In vitro O-Antigen Ligase Assay -- Functional Identification of Bacterial Glucosyltransferase WbdN -- Methods for the Pasteurella Glycosaminoglycan Synthases: Enzymes that Polymerize Hyaluronan, Chondroitin, or Heparosan Chains -- Methods for Measuring Class I Membrane-bound Hyaluronan Synthase Activity -- Creation and Characterization of Glycosyltransferase Mutants of Trypanosoma brucei -- Mannose-6-Phosphate: A Regulator of LLO Destruction -- N-Acetylglucosaminyltransferase (GnT) Assays using Fluorescent Oligosaccharide Acceptor Substrates: GnT-III, IV, V, and IX (GnT-Vb) -- A Method for Determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI Activity -- In vitro Assays of Orphan Glycosyltransferases and their Application to Identify Notch Xylosyltransferases -- In vitro Folding of -1,4Galactosyltransferase and Polypeptide--N-acetylgalactosaminyltransferase from the Inclusion Bodies -- An Assay for 1,6-Fucosyltransferase (FUT8) Activity Based on the HPLC Separation of a Reaction Product with Fluorescence Detection -- Immunodetection of Glycosyltransferases in Gastrointestinal Tissues -- Glycosyltransferases in Chemo-Enzymatic Synthesis of Oligosaccharides -- Tumor Targeting by a Carbohydrate Ligand-Mimicking Peptide -- Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting - SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-step Lectin Affinity Chromatography -- Generation of Monoclonal Antibodies to Native Active Human Glycosyltransferases. 
650 0 |a Biochemistry. 
650 0 |a Analytical chemistry. 
650 0 |a Carbohydrates. 
650 1 4 |a Biochemistry, general. 
650 2 4 |a Analytical Chemistry. 
650 2 4 |a Carbohydrate Chemistry. 
700 1 |a Brockhausen, Inka.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)