Glycosyltransferases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Brockhausen, Inka. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:1st ed. 2013.
Colección:Methods in Molecular Biology, 1022
Materias:
Tabla de Contenidos:
  • Glycan Microarray Screening Assay for Glycosyltransferase Specificities
  • A Fluorescence-Based Assay for Core 1 3Galactosyltransferase (T-synthase) Activity.- Structural and Biochemical Analysis of a Bacterial Glycosyltransferase
  • Study of the Biological Functions of Mucin Type Core 3 O-glycans
  • Generation of Anti-Sulfated Glycan Antibodies using Sulfotransferase-deficient Mice
  • Fluorescent Microscopy as a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in COG Complex Depleted Mammalian Cells
  • A Practical Approach to Reconstruct Evolutionary History of Animal Sialyltransferases and Gain Insights into the Sequence-Function Relationships of Golgi-Glycosyltransferases
  • Fluorescent Lectin Staining of Drosophila Embryos and Tissues to Detect the Spatial Distribution of Glycans during Development
  • Photoaffinity Labeling of Protein O-Mannosyltransferases of the PMT1/PMT2 Subfamily
  • Enzymatic Analysis of the Protein O-glycosyltransferase, Rumi, Acting toward Epidermal Growth Factor-like (EGF) Repeats
  • Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling
  • Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase, and Assay of its Substrate, UDP-GlcNAc
  • In vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases
  • In vitro UDP-sugar:Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection by Thin Layer Chromatography
  • In vitro O-Antigen Ligase Assay
  • Functional Identification of Bacterial Glucosyltransferase WbdN
  • Methods for the Pasteurella Glycosaminoglycan Synthases: Enzymes that Polymerize Hyaluronan, Chondroitin, or Heparosan Chains
  • Methods for Measuring Class I Membrane-bound Hyaluronan Synthase Activity
  • Creation and Characterization of Glycosyltransferase Mutants of Trypanosoma brucei
  • Mannose-6-Phosphate: A Regulator of LLO Destruction
  • N-Acetylglucosaminyltransferase (GnT) Assays using Fluorescent Oligosaccharide Acceptor Substrates: GnT-III, IV, V, and IX (GnT-Vb)
  • A Method for Determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI Activity
  • In vitro Assays of Orphan Glycosyltransferases and their Application to Identify Notch Xylosyltransferases
  • In vitro Folding of -1,4Galactosyltransferase and Polypeptide--N-acetylgalactosaminyltransferase from the Inclusion Bodies
  • An Assay for 1,6-Fucosyltransferase (FUT8) Activity Based on the HPLC Separation of a Reaction Product with Fluorescence Detection
  • Immunodetection of Glycosyltransferases in Gastrointestinal Tissues
  • Glycosyltransferases in Chemo-Enzymatic Synthesis of Oligosaccharides
  • Tumor Targeting by a Carbohydrate Ligand-Mimicking Peptide
  • Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting - SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-step Lectin Affinity Chromatography
  • Generation of Monoclonal Antibodies to Native Active Human Glycosyltransferases.