Glycosyltransferases : Methods and Protocols /
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edition: | 1st ed. 2013. |
| Series: | Methods in Molecular Biology,
1022 |
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Table of Contents:
- Glycan Microarray Screening Assay for Glycosyltransferase Specificities
- A Fluorescence-Based Assay for Core 1 3Galactosyltransferase (T-synthase) Activity.- Structural and Biochemical Analysis of a Bacterial Glycosyltransferase
- Study of the Biological Functions of Mucin Type Core 3 O-glycans
- Generation of Anti-Sulfated Glycan Antibodies using Sulfotransferase-deficient Mice
- Fluorescent Microscopy as a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in COG Complex Depleted Mammalian Cells
- A Practical Approach to Reconstruct Evolutionary History of Animal Sialyltransferases and Gain Insights into the Sequence-Function Relationships of Golgi-Glycosyltransferases
- Fluorescent Lectin Staining of Drosophila Embryos and Tissues to Detect the Spatial Distribution of Glycans during Development
- Photoaffinity Labeling of Protein O-Mannosyltransferases of the PMT1/PMT2 Subfamily
- Enzymatic Analysis of the Protein O-glycosyltransferase, Rumi, Acting toward Epidermal Growth Factor-like (EGF) Repeats
- Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling
- Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase, and Assay of its Substrate, UDP-GlcNAc
- In vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases
- In vitro UDP-sugar:Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection by Thin Layer Chromatography
- In vitro O-Antigen Ligase Assay
- Functional Identification of Bacterial Glucosyltransferase WbdN
- Methods for the Pasteurella Glycosaminoglycan Synthases: Enzymes that Polymerize Hyaluronan, Chondroitin, or Heparosan Chains
- Methods for Measuring Class I Membrane-bound Hyaluronan Synthase Activity
- Creation and Characterization of Glycosyltransferase Mutants of Trypanosoma brucei
- Mannose-6-Phosphate: A Regulator of LLO Destruction
- N-Acetylglucosaminyltransferase (GnT) Assays using Fluorescent Oligosaccharide Acceptor Substrates: GnT-III, IV, V, and IX (GnT-Vb)
- A Method for Determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI Activity
- In vitro Assays of Orphan Glycosyltransferases and their Application to Identify Notch Xylosyltransferases
- In vitro Folding of -1,4Galactosyltransferase and Polypeptide--N-acetylgalactosaminyltransferase from the Inclusion Bodies
- An Assay for 1,6-Fucosyltransferase (FUT8) Activity Based on the HPLC Separation of a Reaction Product with Fluorescence Detection
- Immunodetection of Glycosyltransferases in Gastrointestinal Tissues
- Glycosyltransferases in Chemo-Enzymatic Synthesis of Oligosaccharides
- Tumor Targeting by a Carbohydrate Ligand-Mimicking Peptide
- Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting - SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-step Lectin Affinity Chromatography
- Generation of Monoclonal Antibodies to Native Active Human Glycosyltransferases.