Germinal Centers : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Calado, Dinis Pedro. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2017.
Edition:1st ed. 2017.
Series:Methods in Molecular Biology, 1623
Subjects:
Table of Contents:
  • Analysis of the Germinal Center Reaction in Tissue Sections
  • Detecting Gene Expression in Lymphoid Microenvironments by Laser Microdissection and Quantitative RT-PCR
  • Tracking Plasma Cell Differentiation in Living Mice with 2-Photon Microscopy
  • Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation
  • Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-photon Photoconversion
  • Intravital Microscopy of T-B Cell Interactions in Germinal Centers
  • Identifying Follicular Regulatory T cells by Confocal Microscopy
  • Cytokine Expression by T follicular Helper Cells
  • Follicular Dendritic Cell Isolation and Loading of Immune Complexes
  • Isolation and Characterization of Mouse and Human Follicular Dendritic Cells
  • In-vitro-induced Germinal Center B Cell Culture System
  • CRISPR/Cas9 Mediated In Vitro Mutagenesis in GC-like B Cells
  • Germinal Center Formation with Retrovirally-transduced B Cells for Determining the Role of Specific Molecules In Vivo
  • Characterization of the B Cell Transcriptome by RNA-Binding Proteins using iCLIP
  • Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity
  • Somatic Hypermutation and Affinity Maturation Analysis Using the 4-hydroxy-3-nitrophenyl-acetyl (NP) System
  • Targeting Gene Function in Germinal Center B Cells: A Practical Approach
  • Development of Mouse Model Systems of Germinal Centre Lymphomas
  • The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas
  • Determining the Origin of Human Germinal Center B Cell-derived Malignancies
  • Tracking B-cell Repertoires and Clonal Histories in Normal and Malignant Lymphocytes
  • How to Simulate a Germinal Centre. .