Imaging Gene Expression : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Shav-Tal, Yaron. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:2nd ed. 2019.
Colección:Methods in Molecular Biology, 2038
Materias:
Tabla de Contenidos:
  • Imaging Single mRNA Molecules in Mammalian Cells using an Optimized MS2-MCP System
  • Live-Cell Imaging of Long Non-Coding RNAs using Molecular Beacons
  • Real-Time Observation of Localization and EXpression (ROLEX) System for Live Imaging of the Transcriptional Activity and Nuclear Position of a Specific Endogenous Gene
  • Visualization of Single mRNAs in Live Neurons
  • Lightening Up Gene Activation in Living Drosophila Embryos
  • Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysis
  • Imaging rRNA Methylation in Bacteria by MR-FISH
  • Detection of mRNA Transfer between Mammalian Cells in Co-Culture by Single Molecule Fluorescent In Situ Hybridization (smFISH)
  • Live-Cell Imaging of mRNP-NPC Interactions in Budding Yeast
  • Detection of mRNAs Anchored to the Nuclear Envelope during Export Inhibition in Living Cells
  • Programmable Chromosome Painting with Oligopaints
  • Imaging Chromosome Territory and Gene Loci Positions in Cells Grown on Soft Matrices
  • Targeted Manipulation/Repositioning of Subcellular Structures and Molecules
  • Visualization of Endogenous Transcription Factors in Single Cells using an Antibody Electroporation-Based Imaging Approach
  • Monitoring Transcription Factor Oligomerization in Single Living Cells by Numbers and Brightness Analysis
  • Visualizing Transcription Factor Binding on Mitotic Chromosomes using Single Molecule Live-Cell Imaging
  • Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells.