Microinjection : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Liu, Chengyu. (Editor), Du, Yubin. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2019.
Edition:1st ed. 2019.
Series:Methods in Molecular Biology, 1874
Subjects:
Table of Contents:
  • Microinjection and Micromanipulation: A Historical Perspective
  • Production of Transgenic Mice by Pronuclear Microinjection
  • Transgene Recombineering in Bacterial Artificial Chromosomes
  • Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice
  • Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method
  • Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer
  • Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection
  • Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice
  • Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique
  • Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation
  • Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides
  • Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection
  • The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications
  • The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm
  • Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line
  • Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology
  • Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos
  • Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells
  • Generation of Rabbit Models by Gene Editing Nucleases
  • Production of Genetically Engineered Porcine Embryos by Handmade Cloning
  • Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production
  • Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer
  • Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting
  • Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes
  • Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing
  • Microinjection in Zebrafish for Genome Editing and Functional Studies
  • Microinjection of Marine Fish Eggs
  • Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method
  • Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus
  • Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function.