Microinjection : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
1874 |
| Materias: |
Tabla de Contenidos:
- Microinjection and Micromanipulation: A Historical Perspective
- Production of Transgenic Mice by Pronuclear Microinjection
- Transgene Recombineering in Bacterial Artificial Chromosomes
- Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice
- Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method
- Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer
- Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection
- Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice
- Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique
- Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation
- Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides
- Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection
- The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications
- The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm
- Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line
- Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology
- Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos
- Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells
- Generation of Rabbit Models by Gene Editing Nucleases
- Production of Genetically Engineered Porcine Embryos by Handmade Cloning
- Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production
- Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer
- Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting
- Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes
- Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing
- Microinjection in Zebrafish for Genome Editing and Functional Studies
- Microinjection of Marine Fish Eggs
- Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method
- Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus
- Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function.