Hepatitis C Virus Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Law, Mansun. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1911
Materias:
Tabla de Contenidos:
  • Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus
  • Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV
  • Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR)
  • Establishment of Replication-competent HCV Strain with Minimum Modifications
  • Full-length Open Reading Frame Amplification of Hepatitis C Virus
  • InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones
  • Production and Purification of Cell Culture Hepatitis C Virus
  • Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture
  • Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing
  • A Cell Culture Model for Persistent HCV Infection
  • Expression Cloning of Host Factors Required for the HCV Replication Cycle
  • Application of Deep Mutational Scanning in Hepatitis C Virus
  • Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals
  • Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay
  • Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells
  • Functional Characterization of Apolipoproteins in the HCV Life Cycle
  • Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes
  • Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins
  • Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis
  • Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells
  • Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact
  • Complement Regulation and Immune Evasion by Hepatitis C Virus
  • Direct ex vivo Functional Analysis of HCV-specific T Cells
  • ­Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry
  • Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology. -Isolation of HCV Neutralizing Antibodies by Yeast Display
  • Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA
  • In vitro Neutralization Assay using Cultured Hepatitis C Virus
  • Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp)
  • Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice
  • Mice with Chimeric Human Livers and Their Applications
  • Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies.