MicroRNAs and the Immune System : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Monticelli, Silvia. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edición:1st ed. 2010.
Colección:Methods in Molecular Biology, 667
Materias:
Tabla de Contenidos:
  • Analysis of miRNA Expression: Classic Methods Revisited
  • A Rapid, Quantitative Assay for Direct Detection of MicroRNAs and Other Small RNAs Using Splinted Ligation
  • Normalization of MicroRNA Quantitative RT-PCR Data in Reduced Scale Experimental Designs
  • MicroRNA Detection in Bone Marrow Cells by LNA-FISH
  • Measuring MicroRNA Expression in Size-Limited FACS-Sorted and Microdissected Samples
  • High-Throughput Analysis of miRNAs
  • MicroRNA Cloning from Cells of the Immune System
  • High-Throughput Profiling in the Hematopoietic System
  • Construction of Small RNA cDNA Libraries for Deep Sequencing
  • MicroRNA-Profiling in Formalin-Fixed Paraffin-Embedded Specimens
  • Functional Analysis of miRNAs in the Immune System: Gain-of-Function
  • Expression of miRNAs in Lymphocytes: A Review
  • Mouse Models for miRNA Expression: The ROSA26 Locus
  • Regulation of Monocytopoiesis by MicroRNAs
  • MicroRNA Activity in B Lymphocytes
  • Isolation and Characterization of MicroRNAs of Human Mature Erythrocytes
  • Stable Overexpression of miRNAs in Bone Marrow-Derived Murine Mast Cells Using Lentiviral Expression Vectors
  • Monitoring MicroRNA Activity and Validating MicroRNA Targets by Reporter-Based Approaches
  • Functional Analysis of miRNAs in the Immune System: Loss-of-Function
  • Lentivirus-Mediated Antagomir Expression
  • miRNA Post-Transcriptional Modifications and Mechanisms of Action
  • Solution Structure of miRNA:mRNA Complex
  • MiRNA Editing
  • Bioinformatic Analysis and Target Prediction
  • Computational Prediction of MicroRNA Targets
  • Large-Scale Integration of MicroRNA and Gene Expression Data for Identification of Enriched MicroRNA–mRNA Associations in Biological Systems
  • miRNA and Viruses
  • Identification and Validation of the Cellular Targets of Virus-Encoded MicroRNAs.