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02912nam a22003255i 4500 |
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120118s2012 xxu| s |||| 0|eng d |
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|a 9781617795138
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024 |
7 |
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|a 10.1007/978-1-61779-513-8
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
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|a Mucins :
|b Methods and Protocols /
|c edited by Michael A. McGuckin, David J. Thornton.
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250 |
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|a 1st ed. 2012.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2012.
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300 |
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|a XI, 329 p. 48 illus., 4 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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338 |
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|a online resource
|b cr
|2 rdacarrier
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490 |
1 |
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|a Methods in Molecular Biology,
|v 842
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505 |
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|a Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins -- Gel-forming and Cell-associated Mucins – preparation for Structural and Functional Studies -- Detecting, Visualising and Quantifying Mucins -- Mass Spectrometric Analysis of Mucin Core Proteins -- O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates -- Analysis of Assembly of Secreted Mucins -- MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes -- Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides -- Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides -- O-Glycomics: Profiling and Structural Analysis of Mucin-type O-linked Glycans -- O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry -- Analysing Mucin Degradation -- Assessment of Mucus Thickness and Production In Situ -- Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH -- In vitro Measurements of Mucus Secretion by Colon Explants -- Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function -- Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures -- Assessment of Intracellular Mucin Content In Vivo -- Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo -- Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro.
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650 |
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|a Biochemistry.
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650 |
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|a Proteomics.
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650 |
1 |
4 |
|a Biochemistry, general.
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650 |
2 |
4 |
|a Proteomics.
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700 |
1 |
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|a McGuckin, Michael A.
|e editor.
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700 |
1 |
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|a Thornton, David J.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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