Mucins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: McGuckin, Michael A. (Editor ), Thornton, David J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edición:1st ed. 2012.
Colección:Methods in Molecular Biology, 842
Materias:
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024 7 |a 10.1007/978-1-61779-513-8  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Mucins :  |b Methods and Protocols /  |c edited by Michael A. McGuckin, David J. Thornton. 
250 |a 1st ed. 2012. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2012. 
300 |a XI, 329 p. 48 illus., 4 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 842 
505 0 |a Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins -- Gel-forming and Cell-associated Mucins – preparation for Structural and Functional Studies -- Detecting, Visualising and Quantifying Mucins -- Mass Spectrometric Analysis of Mucin Core Proteins -- O-Glycoprotein Biosynthesis:  Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates -- Analysis of Assembly of Secreted Mucins -- MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes -- Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides -- Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides -- O-Glycomics: Profiling and Structural Analysis of Mucin-type O-linked Glycans -- O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry -- Analysing Mucin Degradation -- Assessment of Mucus Thickness and Production In Situ -- Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH -- In vitro Measurements of Mucus Secretion by Colon Explants -- Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function -- Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures -- Assessment of Intracellular Mucin Content In Vivo -- Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo -- Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro. 
650 0 |a Biochemistry. 
650 0 |a Proteomics. 
650 1 4 |a Biochemistry, general. 
650 2 4 |a Proteomics. 
700 1 |a McGuckin, Michael A.  |e editor. 
700 1 |a Thornton, David J.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)