Mucins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: McGuckin, Michael A. (Editor ), Thornton, David J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edición:1st ed. 2012.
Colección:Methods in Molecular Biology, 842
Materias:
Tabla de Contenidos:
  • Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins
  • Gel-forming and Cell-associated Mucins – preparation for Structural and Functional Studies
  • Detecting, Visualising and Quantifying Mucins
  • Mass Spectrometric Analysis of Mucin Core Proteins
  • O-Glycoprotein Biosynthesis:  Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates
  • Analysis of Assembly of Secreted Mucins
  • MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes
  • Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides
  • Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides
  • O-Glycomics: Profiling and Structural Analysis of Mucin-type O-linked Glycans
  • O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry
  • Analysing Mucin Degradation
  • Assessment of Mucus Thickness and Production In Situ
  • Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH
  • In vitro Measurements of Mucus Secretion by Colon Explants
  • Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function
  • Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures
  • Assessment of Intracellular Mucin Content In Vivo
  • Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo
  • Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro.