|
|
|
|
| LEADER |
02932nam a22003135i 4500 |
| 001 |
000284553 |
| 005 |
20210902202359.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100623s2010 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781603272995
|
| 024 |
7 |
|
|a 10.1007/978-1-60327-299-5
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Matrix Metalloproteinase Protocols /
|c edited by Ian M. Clark.
|
| 250 |
|
|
|a 2nd ed. 2010.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2010.
|
| 300 |
|
|
|a XII, 472 p. :
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 622
|
| 505 |
0 |
|
|a MMPs and TIMPs: An Overview -- Metalloproteases and the Degradome -- Mouse Models of MMP and TIMP Function -- Expression and Purification of MMPs and TIMPs -- Expression of Recombinant MMP-28 in Mammalian Cells -- Expression of Recombinant Matrix Metalloproteinases in Escherichia coli -- Expression of Recombinant ADAMTS in Insect Cells -- Expression and Purification of Membrane-Type MMPs -- Refolding of TIMP-2 from Escherichia coli Inclusion Bodies -- Purification of MMPs and TIMPs -- Detection of MMPs and TIMPs -- Real-Time PCR Expression Profiling of MMPs and TIMPs -- Analysis of the Degradome with the CLIP-CHIP™ Microarray -- In Situ Hybridization for Metalloproteinases and Their Inhibitors -- Immunohistochemistry of MMPs and TIMPs -- Single Nucleotide Polymorphism Genotyping in MMP Genes: The 5? Nuclease Assay -- Assay of MMP and TIMP Activities -- Methods for Studying Activation of Matrix Metalloproteinases -- Assay of Matrix Metalloproteinases Against Matrix Substrates -- Zymography and Reverse Zymography for Detecting MMPs and TIMPs -- In Situ Zymography -- Near-Infrared Optical Proteolytic Beacons for In Vivo Imaging of Matrix Metalloproteinase Activity -- Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan -- In Vitro Model of Cartilage Degradation -- Immunoassays for Collagenase-Mediated Cleavage of Type I and II Collagens -- Collagen Degradation Assays -- Analysis of MMP-Dependent Cell Migration and Invasion -- Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases -- Kinetic Analysis of the Inhibition of Matrix Metalloproteinases: Lessons from the Study of Tissue Inhibitors of Metalloproteinases -- Identification of Cellular MMP Substrates Using Quantitative Proteomics: Isotope-Coded Affinity Tags (ICAT) and Isobaric Tags for Relative and Absolute Quantification (iTRAQ) -- Mechanism-Based Profiling of MMPs.
|
| 650 |
|
0 |
|a Biochemistry.
|
| 650 |
|
0 |
|a Enzymology.
|
| 650 |
1 |
4 |
|a Biochemistry, general.
|
| 650 |
2 |
4 |
|a Science, Humanities and Social Sciences, multidisciplinary.
|
| 650 |
2 |
4 |
|a Enzymology.
|
| 700 |
1 |
|
|a Clark, Ian M.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|