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100623s2010 xxu| s |||| 0|eng d |
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|a 9781603272995
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|a 10.1007/978-1-60327-299-5
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Matrix Metalloproteinase Protocols /
|c edited by Ian M. Clark.
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|a 2nd ed. 2010.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2010.
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|a XII, 472 p. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 622
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|a MMPs and TIMPs: An Overview -- Metalloproteases and the Degradome -- Mouse Models of MMP and TIMP Function -- Expression and Purification of MMPs and TIMPs -- Expression of Recombinant MMP-28 in Mammalian Cells -- Expression of Recombinant Matrix Metalloproteinases in Escherichia coli -- Expression of Recombinant ADAMTS in Insect Cells -- Expression and Purification of Membrane-Type MMPs -- Refolding of TIMP-2 from Escherichia coli Inclusion Bodies -- Purification of MMPs and TIMPs -- Detection of MMPs and TIMPs -- Real-Time PCR Expression Profiling of MMPs and TIMPs -- Analysis of the Degradome with the CLIP-CHIP™ Microarray -- In Situ Hybridization for Metalloproteinases and Their Inhibitors -- Immunohistochemistry of MMPs and TIMPs -- Single Nucleotide Polymorphism Genotyping in MMP Genes: The 5? Nuclease Assay -- Assay of MMP and TIMP Activities -- Methods for Studying Activation of Matrix Metalloproteinases -- Assay of Matrix Metalloproteinases Against Matrix Substrates -- Zymography and Reverse Zymography for Detecting MMPs and TIMPs -- In Situ Zymography -- Near-Infrared Optical Proteolytic Beacons for In Vivo Imaging of Matrix Metalloproteinase Activity -- Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan -- In Vitro Model of Cartilage Degradation -- Immunoassays for Collagenase-Mediated Cleavage of Type I and II Collagens -- Collagen Degradation Assays -- Analysis of MMP-Dependent Cell Migration and Invasion -- Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases -- Kinetic Analysis of the Inhibition of Matrix Metalloproteinases: Lessons from the Study of Tissue Inhibitors of Metalloproteinases -- Identification of Cellular MMP Substrates Using Quantitative Proteomics: Isotope-Coded Affinity Tags (ICAT) and Isobaric Tags for Relative and Absolute Quantification (iTRAQ) -- Mechanism-Based Profiling of MMPs.
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|a Biochemistry.
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|a Enzymology.
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|a Biochemistry, general.
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|a Science, Humanities and Social Sciences, multidisciplinary.
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|a Enzymology.
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|a Clark, Ian M.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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