Matrix Metalloproteinase Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2010.
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| Edición: | 2nd ed. 2010. |
| Colección: | Methods in Molecular Biology,
622 |
| Materias: |
Tabla de Contenidos:
- MMPs and TIMPs: An Overview
- Metalloproteases and the Degradome
- Mouse Models of MMP and TIMP Function
- Expression and Purification of MMPs and TIMPs
- Expression of Recombinant MMP-28 in Mammalian Cells
- Expression of Recombinant Matrix Metalloproteinases in Escherichia coli
- Expression of Recombinant ADAMTS in Insect Cells
- Expression and Purification of Membrane-Type MMPs
- Refolding of TIMP-2 from Escherichia coli Inclusion Bodies
- Purification of MMPs and TIMPs
- Detection of MMPs and TIMPs
- Real-Time PCR Expression Profiling of MMPs and TIMPs
- Analysis of the Degradome with the CLIP-CHIP™ Microarray
- In Situ Hybridization for Metalloproteinases and Their Inhibitors
- Immunohistochemistry of MMPs and TIMPs
- Single Nucleotide Polymorphism Genotyping in MMP Genes: The 5? Nuclease Assay
- Assay of MMP and TIMP Activities
- Methods for Studying Activation of Matrix Metalloproteinases
- Assay of Matrix Metalloproteinases Against Matrix Substrates
- Zymography and Reverse Zymography for Detecting MMPs and TIMPs
- In Situ Zymography
- Near-Infrared Optical Proteolytic Beacons for In Vivo Imaging of Matrix Metalloproteinase Activity
- Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan
- In Vitro Model of Cartilage Degradation
- Immunoassays for Collagenase-Mediated Cleavage of Type I and II Collagens
- Collagen Degradation Assays
- Analysis of MMP-Dependent Cell Migration and Invasion
- Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases
- Kinetic Analysis of the Inhibition of Matrix Metalloproteinases: Lessons from the Study of Tissue Inhibitors of Metalloproteinases
- Identification of Cellular MMP Substrates Using Quantitative Proteomics: Isotope-Coded Affinity Tags (ICAT) and Isobaric Tags for Relative and Absolute Quantification (iTRAQ)
- Mechanism-Based Profiling of MMPs.