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141201s2015 xxu| s |||| 0|eng d |
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|a 9781493922055
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| 024 |
7 |
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|a 10.1007/978-1-4939-2205-5
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Insoluble Proteins :
|b Methods and Protocols /
|c edited by Elena García-Fruitós.
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| 250 |
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|a 1st ed. 2015.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a XVI, 425 p. 72 illus., 41 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1258
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| 505 |
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|a General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins -- Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production -- Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility -- Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification -- Beyond the Cytoplasm of Escherichia coli: Localizing Recombinant Proteins Where You Want Them -- Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates -- Co-Translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems -- Functional Expression of Plant Membrane Proteins in Lactococcus lactis -- High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of “Difficult-to-Express” Secretory Recombinant Proteins -- Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins -- Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins -- Recombinant Glycoprotein Production in Human Cell Lines -- Soluble Recombinant Protein Production in Pseudoaltermonas haloplanktis TAC125 -- A Screening Methodology for Purifying Proteins with Aggregation Problems -- Solubilization and Refolding of Inclusion Body Proteins -- Bacterial Inclusion Body Purification -- Characterization of Intracellular Aggresomes by Fluorescent Microscopy -- Dialysis: A Characterization Method of Aggregation Tendency -- Applications of Mass Spectrometry to the Study of Protein Aggregation -- Insoluble Protein Assemblies Characterized by Fourier Transform Infrared Spectroscopy -- Insoluble Protein Characterization by Circular Dichroism (CD) Spectroscopy and Nuclear Magnetic Resonance (NMR) -- Methods for Characterization of Protein Aggregates -- Predicting the Solubility of Recombinant Proteins in Escherichia coli -- Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts.
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a García-Fruitós, Elena.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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