Insoluble Proteins : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edición: | 1st ed. 2015. |
| Colección: | Methods in Molecular Biology,
1258 |
| Materias: |
Tabla de Contenidos:
- General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins
- Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production
- Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility
- Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification
- Beyond the Cytoplasm of Escherichia coli: Localizing Recombinant Proteins Where You Want Them
- Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates
- Co-Translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems
- Functional Expression of Plant Membrane Proteins in Lactococcus lactis
- High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of “Difficult-to-Express” Secretory Recombinant Proteins
- Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins
- Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins
- Recombinant Glycoprotein Production in Human Cell Lines
- Soluble Recombinant Protein Production in Pseudoaltermonas haloplanktis TAC125
- A Screening Methodology for Purifying Proteins with Aggregation Problems
- Solubilization and Refolding of Inclusion Body Proteins
- Bacterial Inclusion Body Purification
- Characterization of Intracellular Aggresomes by Fluorescent Microscopy
- Dialysis: A Characterization Method of Aggregation Tendency
- Applications of Mass Spectrometry to the Study of Protein Aggregation
- Insoluble Protein Assemblies Characterized by Fourier Transform Infrared Spectroscopy
- Insoluble Protein Characterization by Circular Dichroism (CD) Spectroscopy and Nuclear Magnetic Resonance (NMR)
- Methods for Characterization of Protein Aggregates
- Predicting the Solubility of Recombinant Proteins in Escherichia coli
- Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts.