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140208s2014 xxu| s |||| 0|eng d |
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|a 9781627039680
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| 024 |
7 |
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|a 10.1007/978-1-62703-968-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Homing Endonucleases :
|b Methods and Protocols /
|c edited by David R. Edgell.
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| 250 |
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|a 1st ed. 2014.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a X, 284 p. 60 illus., 39 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1123
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| 505 |
0 |
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|a Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers -- Bioinformatic Identification of Homing Endonucleases and Their Target Sites -- PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes -- Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein -- Mapping Free-Standing Homing Endonuclease Promoters Using 5’RLM-RACE -- PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis -- A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases -- Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection -- A Yeast-Based Recombination Assay for Homing Endonuclease Activity.- Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile -- Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling -- Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries -- Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display -- Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes -- Bioinformatics Identification of Coevolving Residues -- Identification and Analysis of Genomic Homing Endonuclease Target Sites -- Redesigning the Specificity of Protein-DNA Interactions with Rosetta.
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| 650 |
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|a Human genetics.
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| 650 |
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0 |
|a Genetic engineering.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Genetic Engineering.
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| 700 |
1 |
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|a Edgell, David R.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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