Homing Endonucleases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Edgell, David R. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1123
Materias:
Tabla de Contenidos:
  • Homing Endonucleases:  From Genetic Anomalies to Programmable Genomic Clippers
  • Bioinformatic Identification of Homing Endonucleases and Their Target Sites
  • PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes
  • Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein
  • Mapping Free-Standing Homing Endonuclease Promoters Using 5’RLM-RACE
  • PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis
  • A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases
  • Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection
  • A Yeast-Based Recombination Assay for Homing Endonuclease Activity.- Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile
  • Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling
  • Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries
  • Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display
  • Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes
  • Bioinformatics Identification of Coevolving Residues
  • Identification and Analysis of Genomic Homing Endonuclease Target Sites
  • Redesigning the Specificity of Protein-DNA Interactions with Rosetta.