Helicases : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Abdelhaleem, Mohamed M. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edition:1st ed. 2010.
Series:Methods in Molecular Biology, 587
Subjects:
Table of Contents:
  • Helicases: An Overview
  • Fluorescent Biosensors to Investigate Helicase Activity
  • Single-Molecule FRET Analysis of Helicase Functions
  • Kinetics of Motor Protein Translocation on Single-Stranded DNA
  • Experimental and Computational Analysis of DNA Unwinding and Polymerization Kinetics
  • Protein Displacement by Helicases
  • In Vivo Investigation of Protein-Protein Interactions for Helicases Using Tandem Affinity Purification
  • Mapping Genomic Targets of DNA Helicases by Chromatin Immunoprecipitation in Saccharomyces cerevisiae
  • Methods to Study How Replication Fork Helicases Unwind DNA
  • Simple Enzymatic Assays for the In Vitro Motor Activity of Transcription Termination Factor Rho from Escherichia coli
  • Single-Molecule Studies of RecBCD
  • Mutational Analysis of Bloom Helicase
  • In Vivo Analysis of Drosophila BLM Helicase Function During DNA Double-Strand Gap Repair
  • Purification and Characterization of RecQ Helicases of Plants
  • Fluorometric Assay of Hepatitis C Virus NS3 Helicase Activity
  • A Method to Simultaneously Monitor Hepatitis C Virus NS3 Helicase and Protease Activities
  • Computer Modeling of Helicases Using Elastic Network Model
  • Duplex Unwinding with DEAD-Box Proteins
  • Analysis of the RNA Helicase p68 (Ddx5) as a Transcriptional Regulator
  • A Method to Study the Role of DDX3 RNA Helicase in HIV-1
  • Molecular Characterization of Nuclear DNA Helicase II (RNA Helicase A)
  • Regulation of Inter- and Intramolecular Interaction of RNA, DNA, and Proteins by MLE
  • Biochemical Characterization of Human Upf1 Helicase
  • Assays of the Helicase, ATPase, and Exoribonuclease Activities of the Yeast Mitochondrial Degradosome
  • Characterization of the Helicase Activity and Anti-telomerase Properties of Yeast Pif1p In Vitro
  • A Method to Confer Salinity Stress Tolerance to Plants by Helicase Overexpression
  • A Method to Inhibit the Growth of Plasmodium falciparum by Double-Stranded RNA-Mediated Gene Silencing of Helicases.