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150810s2016 xxu| s |||| 0|eng d |
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|a 9781493926688
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| 024 |
7 |
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|a 10.1007/978-1-4939-2668-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Human Embryonic Stem Cell Protocols /
|c edited by Kursad Turksen.
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| 250 |
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|a 3rd ed. 2016.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XIV, 399 p. 68 illus., 40 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1307
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| 505 |
0 |
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|a Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Blastocysts -- Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel -- Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix -- Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate -- Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells -- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR -- Study of Gap Junctions in Human Embryonic Stem Cells -- Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions -- Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells -- Genetic Manipulation of Human Embryonic Stem Cells -- Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System -- Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human Embryonic Stem Cells -- Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation -- Derivation of Endothelial Cells and Pericytes from Human Pluripotent Stem Cells -- Differentiation of Human Embryonic Stem Cells on Periodontal Ligament Fibroblasts -- Derivation of Epithelial Cells from Human Embryonic Stem Cells as an In Vitro Model of Vocal Mucosa -- Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based on Serial Sections -- Derivation of Chondrogenic Cells from Human Embryonic Stem Cells for Cartilage Tissue Engineering -- Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells -- Directed Differentiation of Human Embryonic Stem Cells into Neural Progenitors -- Direct Conversion of Pluripotent Human Embryonic Stem Cells Under Defined Culture Conditions into Human Neuronal or Cardiomyocyte Cell Therapy Derivatives -- Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small Molecule Inhibitor of Src Family Kinases -- Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells -- Accelerated Three-Dimensional Neuroepithelium Formation from Human Embryonic Stem Cells and Its Use for Quantitative Differentiation to Human Retinal Pigment Epithelium -- Efficient Production of Photoreceptor Precursor Cells from Human Embryonic Stem Cells -- Generation of Megakaryocytes and Platelets from Human Pluripotent Stem Cells -- A Simple Protocol for the Generation of Cardiomyocytes from Human Pluripotent Stem Cells.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Turksen, Kursad.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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