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130326s2013 xxu| s |||| 0|eng d |
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|a 9781627033510
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| 024 |
7 |
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|a 10.1007/978-1-62703-351-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Ion Channels :
|b Methods and Protocols /
|c edited by Nikita Gamper.
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| 250 |
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|a 2nd ed. 2013.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XIV, 440 p. 91 illus., 34 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 998
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| 505 |
0 |
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|a Approaches to Cloning of Pain-Related Ion Channel Genes -- Mammalian Expression Systems and Transfection Techniques -- Use of Escherichia coli for the Production and Purification of Membrane Proteins -- Transient Overexpression of Genes in Neurons Using Nucleofection -- Viral Gene Delivery: Optimized Protocol for Production of High Titre Lentiviral Vectors -- Two-Electrode Voltage Clamp -- Conventional Micropipette-Based Patch Clamp Techniques -- Recording of Ion Channel Activity in Planar Lipid Bilayers Experiments -- Recording Macroscopic Currents in Large Patches from Xenopus Oocytes -- Combined Single-Channel and Macroscopic Recording Techniques to Analyze Gating Mechanisms of the Large Conductance Ca2+ and Voltage Activated (BK) Potassium Channel -- Perforated Whole Cell Patch Clamp Recording -- Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons -- Automated Planar Patch-Clamp -- Recording Single-Channel Currents Using “Smart Patch-Clamp” Technique -- Using Total Internal Reflection Fluorescence Microscopy to Observe Ion channel Trafficking and Assembly -- Förster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells -- The Use of Dansyl-Calmodulin to Study Interactions with Channels and Other Proteins -- Imaging and Quantification of Recycled KATP Channels -- Generation of Antibodies that Are Externally-Acting Isoform-Specific Inhibitors of Ion Channels -- Site-Directed Mutagenesis to Study the Structure-Function Relationships of Ion Channels -- Cysteine-Based Cross-Linking Approach to Study Inter-Domain Interactions in Ion Channel Functions -- Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-Ray Crystallography -- Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy -- Recording Dendritic Ion Channel Properties and Function from Cortical Neurons -- M Current Recording from Acute DRG Slices -- Studying Ion Channels in Human Erythrocytes by Direct and Indirect Means -- Recording Ion Channels in Isolated, Split-Opened Tubules -- Single Channel Analysis of TRPC Channels in the Podocytes of Freshly Isolated Glomeruli -- Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons -- Patch Clamping Drosophila Sensory Neurons -- Production and Validation of Recombinant Adeno-Associated Virus for Channelrhodopsin Expression in Neurons -- Optical Control of Ligand-Gated Ion Channels.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Cell membranes .
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Membrane Biology.
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| 700 |
1 |
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|a Gamper, Nikita.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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