Ion Channels : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edición: | 2nd ed. 2013. |
| Colección: | Methods in Molecular Biology,
998 |
| Materias: |
Tabla de Contenidos:
- Approaches to Cloning of Pain-Related Ion Channel Genes
- Mammalian Expression Systems and Transfection Techniques
- Use of Escherichia coli for the Production and Purification of Membrane Proteins
- Transient Overexpression of Genes in Neurons Using Nucleofection
- Viral Gene Delivery: Optimized Protocol for Production of High Titre Lentiviral Vectors
- Two-Electrode Voltage Clamp
- Conventional Micropipette-Based Patch Clamp Techniques
- Recording of Ion Channel Activity in Planar Lipid Bilayers Experiments
- Recording Macroscopic Currents in Large Patches from Xenopus Oocytes
- Combined Single-Channel and Macroscopic Recording Techniques to Analyze Gating Mechanisms of the Large Conductance Ca2+ and Voltage Activated (BK) Potassium Channel
- Perforated Whole Cell Patch Clamp Recording
- Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons
- Automated Planar Patch-Clamp
- Recording Single-Channel Currents Using “Smart Patch-Clamp” Technique
- Using Total Internal Reflection Fluorescence Microscopy to Observe Ion channel Trafficking and Assembly
- Förster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells
- The Use of Dansyl-Calmodulin to Study Interactions with Channels and Other Proteins
- Imaging and Quantification of Recycled KATP Channels
- Generation of Antibodies that Are Externally-Acting Isoform-Specific Inhibitors of Ion Channels
- Site-Directed Mutagenesis to Study the Structure-Function Relationships of Ion Channels
- Cysteine-Based Cross-Linking Approach to Study Inter-Domain Interactions in Ion Channel Functions
- Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-Ray Crystallography
- Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy
- Recording Dendritic Ion Channel Properties and Function from Cortical Neurons
- M Current Recording from Acute DRG Slices
- Studying Ion Channels in Human Erythrocytes by Direct and Indirect Means
- Recording Ion Channels in Isolated, Split-Opened Tubules
- Single Channel Analysis of TRPC Channels in the Podocytes of Freshly Isolated Glomeruli
- Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons
- Patch Clamping Drosophila Sensory Neurons
- Production and Validation of Recombinant Adeno-Associated Virus for Channelrhodopsin Expression in Neurons
- Optical Control of Ligand-Gated Ion Channels.