In Situ Detection of DNA Damage : Methods and Protocols /

In Situ Detection of DNA Damage : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Didenko, Vladimir V. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2002.
Edition:1st ed. 2002.
Series:Methods in Molecular Biology, 203
Subjects:
Human genetics.
Cytology.
Human Genetics.
Cell Biology.
Table of Contents:
  • Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay)
  • Labeling DNA Damage with Terminal Transferase
  • TUNEL Assay
  • Electron Microscopic Detection of DNA Damage Labeled by TUNEL
  • Quantitative Differentiation of Both Free 3? OH and 5? OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron Microscopy
  • Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching Techniques
  • In Situ Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning Cytometry
  • Labeling DNA Breaks Using DNA Polymerase I or its Klenow Fragment
  • DNA Damage Detection Using DNA Polymerase I or its Klenow Fragment
  • Labeling DNA Breaks In Situ by Klenow Enzyme
  • In Situ Nick Translation at the Electron Microscopic Level
  • Labeling DNA Breaks Using Ligase
  • In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections
  • Detection of Specific Double-Strand DNA Breaks and Apoptosis In Situ Using T4 DNA Ligase
  • In Situ Detection of Double-Strand DNA Breaks with Terminal 5?OH Groups
  • Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related Techniques
  • The Comet Assay
  • The Comet Assay
  • Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assays
  • Application of FISH to Detect DNA Damage
  • Detection of Modified Bases and AP Sites in DNA
  • Simultaneous In Situ Detection of DNA Fragmentation and RNA/DNA Oxidative Damage Using TUNEL Assay and Immunohistochemical Labeling for 8-Hydroxy-2?-Deoxyguanosine (8-OHdG)
  • The In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking Termini
  • Indirect and General Markers of DNA Damage
  • Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage
  • Ultrasound Imaging of Apoptosis
  • p53 Induction as an Indicator of DNA Damage
  • Detection of Caspases Activation In Situ by Fluorochrome-Labeled Inhibitors of Caspases (FLICA).

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