Human Airway Inflammation : Sampling Techniques and Analytical Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Rogers, Duncan F. (Editor ), Donnelly, Louise E. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2001.
Edición:1st ed. 2001.
Colección:Methods in Molecular Medicine, 56
Materias:
Tabla de Contenidos:
  • Airway Inflammation and Remodeling in Asthma
  • Airway Inflammation and Remodeling in Asthma
  • Sample Collection
  • Biopsy Techniques
  • Bronchoalveolar Lavage (BAL)
  • Nasal Secretions and Exudations
  • Sample Collection
  • Induced Sputum
  • Induced Sputum
  • Measurement of Exhaled Nitric Oxide and Carbon Monoxide
  • Measurement of Exhaled Hydrocarbons
  • Breath Condensate as a Vehicle for Collection of Inflammatory Mediators, Especially Hydrogen Peroxide
  • Cell Isolation and Culture
  • Airway Epithelial Cells (Primaries vs Cell Lines)
  • Isolation and Culture of Human Alveolar Type II Pneumocytes
  • Pulmonary Artery Endothelial Cells
  • Isolation and Culture of Human Airway Smooth Muscle Cells
  • Isolation and Purification of Human Mast Cells and Basophils
  • Neutrophils
  • Macrophages
  • Collection, Separation, and Activation of Human T Lymphocytes
  • Eosinophils
  • Gene Expression/Regulation
  • Analysis of Gene Expression
  • Analysis of Gene Expression
  • The Technique of In Situ Hybridization
  • Measurement of Airway Mucin Gene Expression
  • Transcription Factors and Inflammatory Gene Regulation
  • Mediators
  • Inflammatory Mediators in Spontaneously Produced Sputum
  • Flow Cytometry
  • Measurement of Granulocyte Pharmacodynamics in Whole Blood by Flow Cytometry
  • Current In Vitro Models of Leukocyte Migration
  • Tracing Intracellular Mediator Storage and Mobilization in Eosinophils
  • Measurement of Metalloproteinases
  • Immunocytochemistry and Immunohistochemistry
  • The Measurement of Cysteinyl Leukotrienes in Urine
  • Quantitative Analysis of Cyclooxygenase Metabolites of Arachidonic Acid
  • Quantitative Analysis of F2 Isoprostanes.