Hydrocarbon and Lipid Microbiology Protocols : Genetic, Genomic and System Analyses of Pure Cultures /
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| Other Authors: | , , |
| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
2017.
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| Edition: | 1st ed. 2017. |
| Series: | Springer Protocols Handbooks,
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| Subjects: |
Table of Contents:
- Introduction
- Broadening the SEVA plasmid repertoire to facilitate genomic editing of Gram-negative bacteria
- Protocols on regulation of gene expression
- Ultra-high-throughput transposon scanning of bacterial genomes
- Knock-in-leave-behind (KILB): Genetic grafting of protease-cleaving sequences into permissive sites of proteins with a Tn5-based transposition system
- Deep sequencing to study microbial transcriptomic responses to hydrocarbon degradation/production/stress
- Shotgun proteomics for hydrocarbon microbiology
- Interactomic characterization of membrane-associated mega-complexes for the anaerobic respiration in Pseudomonas aeruginosa
- Lipidomic analysis of bacteria by thin layer chromatography and liquid chromatography/mass spectrometry
- Accurate microbial genome annotation using an integrated and user-friendly environment for community expertise of gene functions: the MicroScope platform
- Approaches for displaying complete transcriptomes of environmental bacteria
- A practical protocol for integration of transcriptomics data into genome-scale metabolic reconstructions
- GC-MS based determination of mass isotopomer distributions for 13C-based metabolic flux analysis
- Analysis of the hierarchical and metabolic regulation of flux through metabolic pathways.