Listeria monocytogenes : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Jordan, Kieran. (Editor ), Fox, Edward M. (Editor ), Wagner, Martin. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1157
Materias:
Tabla de Contenidos:
  • Sampling the Production/Processing Environment for Listeria
  • Traditional Methods for Isolation of Listeria monocytogenes
  • Confirmation of Isolates of Listeria by Conventional and Real-Time PCR
  • Serotype Assignment by Sero-Agglutination, ELISA and PCR
  • Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes
  • Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes
  • Ribotyping and Automated Ribotyping of Listeria monocytogenes
  • Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes
  • High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray
  • Analysis of Listeria monocytogenes Subproteomes
  • The Listeria Cell Wall and Associated Carbohydrate Polymers
  • Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria
  • Virulence Characterization of Listeria monocytogenes
  • Internalization Assays for Listeria monocytogenes
  • Extraction and Analysis of Plasmid DNA from Listeria monocytogenes
  • Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method
  • Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes
  • Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR
  • Genome Sequencing of Listeria monocytogenes
  • Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels
  • Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers
  • Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique
  • Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments.