Matrix Metalloproteases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Galea, Charles A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1579
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-6863-3
Tabla de Contenidos:
  • Expression and Purification of Matrix Metalloproteinases in Escherichia coli
  • Expression and Purification of a Matrix Metalloprotease Transmembrane Domain in Escherichia coli
  • Heterologous Expression of the Astacin Protease Meprin b in Pichia pastoris
  • Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction
  • Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and other Prospective Peripheral Membrane Proteins
  • Using Small Angle X-ray Scattering (SAXS) to Characterise the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs)
  • Molecular Dynamics Studies of Matrix Metalloproteases
  • Determining the Substrate Specificity of Matrix Metalloproteases using Fluorogenic Peptide Substrates
  • Time-resolved Analysis of Matrix Metalloproteinase Substrates in Complex Samples
  • Identification of Protease Cleavage Sites by Charge-Based Enrichment of Protein N-termini
  • Mapping the Substrate Recognition Landscapes of Metalloproteases using Comprehensive Mutagenesis
  • Detection of Matrix Metalloproteinases by Zymography
  • Imaging Matrix Metalloproteases in Spontaneous Colon Tumors: Validation by Correlation with Histopathology
  • Virtual High-throughput Screening for Matrix Metalloproteinase Inhibitors
  • Computational Approaches to Matrix Metalloprotease Drug Design
  • A Simple, Adaptable Blood-Brain Barrier Cell Model for Screening Matrix Metalloproteinase Inhibitor Functionality
  • Matrix Metalloproteases as Biomarkers of Disease. .