Kinase Screening and Profiling : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Zegzouti, Hicham. (Editor ), Goueli, Said A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1360
Materias:
Tabla de Contenidos:
  • HTRF Kinase Assay Development and Methods in Inhibitor Characterization.- Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening.- Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy
  • Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase and PI5P4Kα Lipid Kinase.- Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity.- Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting Assay.- A High-Throughput Radiometric Kinase Assay
  • A High Content Assay to Screen for Modulators of EGFR Function.- Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays
  • From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors
  • Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells.- Characterization of an Engineered Src Kinase to Study Src Signaling and Biology
  • Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase Substrates
  • Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays
  • Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries.