Kinase Screening and Profiling : Methods and Protocols /
| Corporate Author: | |
|---|---|
| Other Authors: | , | 
| Format: | eBook | 
| Language: | English | 
| Published: | 
      New York, NY :
        Springer New York : Imprint: Humana,
    
      2016.
     | 
| Edition: | 1st ed. 2016. | 
| Series: | Methods in Molecular Biology,
              1360             | 
| Subjects: | 
                Table of Contents: 
            
                  - HTRF Kinase Assay Development and Methods in Inhibitor Characterization.- Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening.- Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy
 - Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase and PI5P4Kα Lipid Kinase.- Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity.- Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting Assay.- A High-Throughput Radiometric Kinase Assay
 - A High Content Assay to Screen for Modulators of EGFR Function.- Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays
 - From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors
 - Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells.- Characterization of an Engineered Src Kinase to Study Src Signaling and Biology
 - Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase Substrates
 - Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays
 - Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries.