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Human Papillomaviruses : Methods and Protocols /

Human Papillomaviruses : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Davy, Clare. (Editor), Doorbar, John. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2006.
Edition:1st ed. 2006.
Series:Methods in Molecular Medicine, 119
Subjects:
Medical microbiology.
Infectious diseases.
Medical Microbiology.
Infectious Diseases.
Table of Contents:
  • Identification of New Papillomavirus Types
  • Identification of HPV Variants
  • Histochemical Analysis of Cutaneous HPV-Associated Lesions
  • Histological Analysis of Cervical Intraepithelial Neoplasia
  • Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections
  • Detection and Quantitation of HPV Gene Expression Using Real-Time PCR
  • Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers
  • Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN
  • HPV DNA Detection and Typing in Cervical Scrapes
  • HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions
  • Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies
  • Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic "Raft" Cultures
  • Differentiation of HPV-Containing Cells Using Organotypic "Raft" Culture or Methylcellulose
  • Propagation of Infectious, High-Risk HPV in Organotypic "Raft" Culture
  • Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic "Raft" Cultures
  • The HPV Xenograft Severe Combined Immunodeficiency Mouse Model
  • The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease
  • Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology
  • Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae
  • Analysis of the Regulation of Viral Transcription
  • Analysis of HPV Transcription by RPA
  • Analysis of Regulatory Motifs Within HPV Transcripts
  • Detection of HPV Transcripts by Nested RT-PCR
  • Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays
  • Detection and Quantitation of HPV DNA Replication by Southern Blotting and Real-time PCR
  • Analysis of E7/Rb Associations
  • Transformation Assays for HPV Oncoproteins
  • Analysis of Adeno-Associated Virus and HPV Interaction
  • In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins
  • Measuring the Induction or Inhibition of Apoptosis by HPV Proteins
  • Codon Optimization of Papillomavirus Genes
  • Generation of HPV Pseudovirions Using Transfection and Their Use in Neutralization Assays
  • Generation and Applications of HPV Pseudovirions Using Vaccinia Virus.

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