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100715s2010 xxu| s |||| 0|eng d |
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|a 9781597453240
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|a 10.1007/978-1-59745-324-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Immunocytochemical Methods and Protocols /
|c edited by Constance Oliver, Maria Célia Jamur.
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| 250 |
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|a 3rd ed. 2010.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2010.
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| 300 |
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|a XII, 416 p. 44 illus., 4 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 588
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| 505 |
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|a Antibody Preparation -- Overview of Antibodies for Immunochemistry -- to the Purification of Antibodies -- Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration -- Antibody Purification: Ion-Exchange Chromatography -- Antibody Purification: Affinity Chromatography – Protein A and Protein G Sepharose -- Conjugation of Fluorochromes to Antibodies -- Biotinylation of Antibodies -- Tissue Preparation for Light Microscopic Analysis -- Cell Fixatives for Immunostaining -- Permeabilization of Cell Membranes -- Preparation of Frozen Sections for Analysis -- Processing of Cytological Specimens -- Processing of Tissue Culture Cells -- Processing of Tissue Specimens -- Heat-Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections -- Bright Field Detection Systems -- Fluorochromes: Properties and Characteristics -- Direct Immunofluorescent Labeling of Cells -- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescent Visualization of Macromolecules in Drosophila Whole Mounts -- Overview of Conventional Fluorescence Photomicrography -- Overview of Confocal Microscopy -- Overview of Laser Microbeam Applications as Related to Antibody Targeting -- Immuno-Laser Capture Microdissection of Rat Brain Neurons for Real Time Quantitative PCR -- Overview of Antigen Detection Through Enzymatic Activity -- The Peroxidase–Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods -- The Avidin–Biotin Complex (ABC) Method and Other Avidin–Biotin Binding Methods -- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue -- Multiple Antigen Immunostaining Procedures -- Immunoenzymatic Quantitative Analysis of Antigens Expressed on the Cell Surface (Cell-ELISA) -- Use of Immunogold with Silver Enhancement -- Fluorescence-Activated Cell Sorter (FACS) Analyses -- Overview of Flow Cytometry and Fluorescent Probes for Flow Cytometry -- Tissue Disaggregation -- Indirect Immunofluorescent Labeling of Viable Cells -- Indirect Immunofluorescent Labeling of Fixed Cells -- Fluorescent Labeling of DNA -- Deparaffinization and Processing of Pathologic Material -- Colloidal Gold Detection Systems for Electron Microscopic Analysis -- Fixation and Embedding -- Preparation of Colloidal Gold -- Conjugation of Colloidal Gold to Proteins -- Colloidal Gold/Streptavidin Methods -- Pre-embedding Labeling Methods -- Postembedding Labeling Methods -- The Clinical Laboratory -- The Clinical Immunohistochemistry Laboratory: Regulations and Troubleshooting Guidelines.
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| 650 |
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|a Immunology.
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| 650 |
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|a Pathology.
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| 650 |
1 |
4 |
|a Immunology.
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| 650 |
2 |
4 |
|a Pathology.
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| 700 |
1 |
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|a Oliver, Constance.
|e editor.
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| 700 |
1 |
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|a Jamur, Maria Célia.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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