Hepatitis C : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Tang, Hengli. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:2nd ed. 2009.
Colección:Methods in Molecular Biology, 510
Materias:
Tabla de Contenidos:
  • Detection and Quantification of HCV
  • Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays
  • Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen
  • Immunohistochemical Detection of HCV Proteins in Liver Tissue
  • HCV Nomenclature, Genotyping, and Quasispecies
  • Nomenclature and Numbering of the Hepatitis C Virus
  • A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates
  • Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry
  • HCV Replication in Vitro
  • Purification and Crystallization of NS5A Domain I of Hepatitis C Virus
  • NS5A Phosphorylation and Hyperphosphorylation
  • Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies
  • Structural and Functional Analysis of the HCV p7 Protein
  • HCV Replicons: Overview and Basic Protocols
  • Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA
  • Studying HCV RNA Synthesis In Vitro with Replication Complexes
  • Proteomics Study of the Hepatitis C Virus Replication Complex
  • Investigation of the Hepatitis C Virus Replication Complex
  • Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease
  • Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay
  • High-Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System
  • Inhibition of HCV Replication by Small Interfering RNA
  • HCV Entry and Infection
  • Cellular Receptors and HCV Entry
  • Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp)
  • Screening of Small-Molecule Compounds as Inhibitors of HCV Entry
  • Isolation of JFH-1 Strain and Development of an HCV Infection System
  • Measuring HCV Infectivity Produced in Cell Culture and In Vivo
  • Genotype 1a HCV (H77S) Infection System
  • Full-Length Infectious HCV Chimeras
  • Adaptation of the Hepatitis C Virus to Cell Culture
  • Primary Human Hepatocyte Culture for HCV Study
  • In Vivo Study of HCV in Mice with Chimeric Human Livers
  • Immunity and Modeling
  • Determination of HCV-Specific T-Cell Activity
  • Analysis of HCV-Specific T Cells by Flow Cytometry
  • Detection of Neutralizing Antibodies with HCV Pseudoparticles (HCVpp)
  • Mathematical Modeling of HCV Infection and Treatment.