Herpes Simplex Virus : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Diefenbach, Russell J. (Editor ), Fraefel, Cornel. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1144
Materias:
Tabla de Contenidos:
  • HSV-1 Biology and Life Cycle
  • Herpes Simplex Virus Growth, Preparation, and Assay
  • Isolation of Herpes Simplex Virus Nucleocapsid DNA
  • Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes
  • Engineering HSV-1 Vectors for Gene Therapy
  • Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors
  • HSV-1 Amplicon Vectors as Genetic Vaccines
  • Modification of HSV-1 to an Oncolytic Virus
  • Herpes Simplex Virus Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo
  • Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals
  • Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation
  • Characterization of Extracellular HSV-1 Virions by Proteomics
  • A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors
  • Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein-Protein Interactions
  • Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy
  • Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication
  • Expression, Purification and Crystallization of HSV-1 Glycoproteins for Structure Determination
  • Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components
  • In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells
  • HSV-1 Protein Expression Using Recombinant Baculoviruses
  • The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines.