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130217s2013 xxu| s |||| 0|eng d |
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|a 9781627032902
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024 |
7 |
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|a 10.1007/978-1-62703-290-2
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Immune Homeostasis :
|b Methods and Protocols /
|c edited by Andrew L. Snow, Michael J. Lenardo.
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250 |
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|a 1st ed. 2013.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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300 |
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|a XI, 215 p. 32 illus., 11 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 979
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|a Quantitating Lymphocyte Programmed Cell Death (PCD) In Vitro Using Simple Kill Assays -- Fluoresence-Activated Cell Sorting (FACS)-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death (RICD) in Activated, Primary Human T Cells -- Evaluation of IL-2-Withdrawal Induced Apoptosis in Human T Lymphocytes -- Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique -- Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation -- Analyses of Programmed Cell Death in Dendritic Cells -- Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity -- Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers -- Homeostatic Proliferation of Mature T Cells -- Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers -- Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis -- Molecular Measurement of T Cell Receptor Excision Circles -- Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis -- Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders -- Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP) -- In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells.
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|a Immunology.
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650 |
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4 |
|a Immunology.
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700 |
1 |
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|a Snow, Andrew L.
|e editor.
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700 |
1 |
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|a Lenardo, Michael J.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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