Immune Homeostasis : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Snow, Andrew L. (Editor ), Lenardo, Michael J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:1st ed. 2013.
Colección:Methods in Molecular Biology, 979
Materias:
Tabla de Contenidos:
  • Quantitating Lymphocyte Programmed Cell Death (PCD) In Vitro Using Simple Kill Assays
  • Fluoresence-Activated Cell Sorting (FACS)-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death (RICD) in Activated, Primary Human T Cells
  • Evaluation of IL-2-Withdrawal Induced Apoptosis in Human T Lymphocytes
  • Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique
  • Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation
  • Analyses of Programmed Cell Death in Dendritic Cells
  • Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity
  • Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers
  • Homeostatic Proliferation of Mature T Cells
  • Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers
  • Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis
  • Molecular Measurement of T Cell Receptor Excision Circles
  • Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis
  • Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders
  • Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP)
  • In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells.