Hematopoietic Stem Cell Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Bunting, Kevin D. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edition:2nd ed. 2008.
Series:Methods in Molecular Biology, 430
Subjects:
Table of Contents:
  • Overview
  • The Expanding Tool Kit for Hematopoietic Stem Cell Research
  • Stem Cell Enrichment and Analysis
  • Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties
  • Mobilization of Hematopoietic Stem and Progenitor Cells in Mice
  • Transmigration of Human CD34+ Cells
  • Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells
  • Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry
  • In Vitro Assays and Differentiation
  • In Vitro Hematopoietic Differentiation of Murine Embryonic Stem Cells
  • Hematopoietic Development of Human Embryonic Stem Cells in Culture
  • In Vitro Human T Cell Development Directed by Notch-Ligand Interactions
  • In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C
  • Transplantation Assays for Mouse and Human HSC
  • Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection
  • Hematopoietic Stem Cell Transplant into Non-Myeloablated W/W v Mice to Detect Steady-State Engraftment Defects
  • Isolation and Functional Characterization of Side Population Stem Cells
  • Transplantation of Chimeric Fetal Liver to Study Hematopoiesis
  • Immunodeficient Mouse Models to Study Human Stem Cell-Mediated Tissue Repair
  • Genetic Modification of HSCs and Imaging Engraftment
  • Retroviral Transduction of Murine Hematopoietic Stem Cells
  • Lentiviral Gene Transduction of Mouse and Human Stem Cells
  • Retroviral Integration Site Analysis in Hematopoietic Stem Cells
  • A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in Stem Cells
  • Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models.