JAK-STAT Signalling : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edición: | 1st ed. 2013. |
| Colección: | Methods in Molecular Biology,
967 |
| Materias: |
Tabla de Contenidos:
- Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
- Co-Immunoprecipitation Protocol to Investigate Cytokine Receptor Associated Proteins e.g. Janus Kinases or Other Associated Signaling Proteins
- In Vitro JAK Kinase Activity and Inhibition Assays
- Quantitative Analysis of JAK Binding using Isothermal Titraion Calorimetry and Surface Plasmon Resonance
- Determination of Protein Turnover Rates in the JAK/STAT Pathway using a Radioactive Pulse-Chase Approach
- Designing RNAi Screens to Identify JAK/STAT Pathway Components
- The Use of JAK-Specific Inhibitors as Chemical Biology Tools
- Methods for Detecting Mutations in the Human JAK2 Gene
- Detection of Activated STAT Proteins
- Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential Pitfalls Arising from the Use of Detergents
- Flow Cytometric Analysis of STAT Phosphorylation
- Acetylation of Endogenous STAT Proteins
- Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining
- Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging
- Characterization of STAT Self-Association by Analytical Ultracentrifugation
- Constitutively Active STAT5 Constructs
- Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR
- Detection of Endogenous SOCS1 and SOCS3 Proteins by Immunoprecipitation and Western Blot Analysis
- In vitro Ubiquitination of Cytokine Signaling Components
- Production and Crystallization of Recombinant JAK Proteins
- Bacterial Expression, Purification and Crystallization of Tyrosine Phosphorylated STAT Proteins.