Molecular and Cell Biology Methods for Fungi /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2010.
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| Edición: | 1st ed. 2010. |
| Colección: | Methods in Molecular Biology,
638 |
| Materias: |
Tabla de Contenidos:
- Fungal Transformation and Gene Knockout
- Protoplast Transformation of Filamentous Fungi
- Electroporation and Agrobacterium-Mediated Spore Transformation
- High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains
- Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi
- DelsGate: A Robust and Rapid Method for Gene Deletion
- Gene Silencing for Functional Analysis: Assessing RNAi as a Tool for Manipulation of Gene Expression
- Detection and Quantification of Fungi
- Analysis of Fungal Gene Expression by Real Time Quantitative PCR
- Identification of Differentially Expressed Fungal Genes In Planta by Suppression Subtraction Hybridization
- Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software
- Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR
- Application of Laser Microdissection to Study Plant-Fungal Pathogen Interactions
- Multiplex Gene Expression Analysis by TRAC in Fungal Cultures
- Amplification of Fungal Genomes Using Multiple Displacement Amplification
- Microscopy and Protein Analysis
- Biochemical Methods Used to Study the Gene Expression and Protein Complexes in the Filamentous Fungus Neurospora crassa
- Measuring Protein Kinase and Sugar Kinase Activity in Plant Pathogenic Fusarium Species
- A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae
- A Method to Visualize the Actin and Microtubule Cytoskeleton by Indirect Immunofluorescence
- Fluorescence In Situ Hybridization for Molecular Cytogenetic Analysis in Filamentous Fungi
- Live-Cell Imaging of Microtubule Dynamics in Hyphae of Neurospora crassa
- Methods to Detect Apoptotic-Like Cell Death in Filamentous Fungi
- Evaluation of Antifungal Susceptibility Using Flow Cytometry
- Preparation of Fungi for Ultrastructural Investigations and Immunogoldlabelling
- Split-EGFP Screens for the Detection and Localisation of Protein-Protein Interactions in Living Yeast Cells.