In Vitro Neurotoxicology : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Costa, Lucio G. (Editor ), Giordano, Gennaro. (Editor ), Guizzetti, Marina. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 758
Materias:
Tabla de Contenidos:
  • In Vitro Neurotoxicology: An Introduction
  • Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies
  • Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures
  • Microglia Cell Culture: A Primer for the Novice
  • Neural Stem Cells for Developmental Neurotoxicity Studies
  • Preparation, Maintenance, and Use of Serum-Free Aggregating Brain Cell Cultures
  • Neurospheres as a Model for Developmental Neurotoxicity Testing
  • Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures
  • Glia-Neuron Sandwich Co-Cultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity
  • In Vitro Models to Study the Blood Brain Barrier
  • Measurements of Cell Death in Neuronal and Glial Cells
  • Measurements of Neuronal Apoptosis
  • Measurement of Isoprostanes as Markers of Oxidative Stress
  • Assessment of Glutathione Homeostasis
  • Assessing Neuronal Bioenergetic Status
  • Fluorescent Assessment of Intracellular Calcium in Individual Cells
  • Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures
  • Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues
  • JNK3-Mediated Apoptotic Cell Death in Primary Dopaminergic Neurons
  • Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration
  • Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo
  • Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons
  • Quantitative Assessment of Neurite Outgrowth in PC12 Cells
  • Measurements of Astrocyte Proliferation
  • Quantification of Synaptic Structure Formation in Co-Cultures of Astrocytes and Hippocampal Neurons
  • Volume Measurements in Cultured Primary Astrocytes
  • Assessment of Cholesterol Homeostasis in Astrocytes and Neurons
  • Dose-Response or Dose-Effect Curves in In Vitro Experiments and Their Use to Study Combined Effects of Neurotoxicants.