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03282nam a22003135i 4500 |
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000287516 |
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20201012085827.0 |
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cr nn 008mamaa |
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181026s2019 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493988372
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| 024 |
7 |
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|a 10.1007/978-1-4939-8837-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Phytoplasmas :
|b Methods and Protocols /
|c edited by Rita Musetti, Laura Pagliari.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XI, 362 p. 75 illus., 64 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1875
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| 505 |
0 |
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|a Phytoplasmas: An Introduction -- Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques -- Phytoplasma Transmission: Insect Rearing and Infection Protocols -- Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors -- Symptoms of Phytoplasma Diseases -- Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas -- Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene -- PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas -- Real-time PCR Protocol for Phytoplasma Detection and Quantification -- Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves -- A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas -- One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas -- A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification -- Quantitative Analysis with Droplet Digital PCR -- Rapid Sample Preparation and LAMP for Phytoplasma Detection -- Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly -- Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of ‘Flavescence Dorée’ -- Transcriptomic Analyses of Phytoplasmas -- Transcriptomic Analyses of Phytoplasmas -- Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study -- Collection of Phloem Sap in Phytoplasma-infected Plants -- DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas -- Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas -- Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC) -- Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants -- Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants. .
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| 650 |
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|a Plant science.
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| 650 |
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0 |
|a Botany.
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| 650 |
1 |
4 |
|a Plant Sciences.
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| 700 |
1 |
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|a Musetti, Rita.
|e editor.
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| 700 |
1 |
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|a Pagliari, Laura.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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