Phytoplasmas : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Musetti, Rita. (Editor ), Pagliari, Laura. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1875
Materias:
Tabla de Contenidos:
  • Phytoplasmas: An Introduction
  • Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques
  • Phytoplasma Transmission: Insect Rearing and Infection Protocols
  • Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors
  • Symptoms of Phytoplasma Diseases
  • Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas
  • Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene
  • PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas
  • Real-time PCR Protocol for Phytoplasma Detection and Quantification
  • Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves
  • A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas
  • One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas
  • A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification
  • Quantitative Analysis with Droplet Digital PCR
  • Rapid Sample Preparation and LAMP for Phytoplasma Detection
  • Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly
  • Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of ‘Flavescence Dorée’
  • Transcriptomic Analyses of Phytoplasmas
  • Transcriptomic Analyses of Phytoplasmas
  • Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study
  • Collection of Phloem Sap in Phytoplasma-infected Plants
  • DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas
  • Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas
  • Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC)
  • Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants
  • Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants. .