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100427s2010 xxu| s |||| 0|eng d |
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|a 9781603274128
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| 024 |
7 |
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|a 10.1007/978-1-60327-412-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Protein Secretion :
|b Methods and Protocols /
|c edited by Anastassios Economou.
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| 250 |
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|a 1st ed. 2010.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2010.
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| 300 |
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|a XV, 426 p. 74 illus., 2 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 619
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| 505 |
0 |
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|a The Extraordinary Diversity of Bacterial Protein Secretion Mechanisms -- In Vitro and In Vivo Approaches to Studying the Bacterial Signal Peptide Processing -- Membrane Insertion of Small Proteins -- Membrane Protein Insertion in E. coli -- Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition -- In Vivo Analysis of Protein Translocation to the Escherichia coli Periplasm -- Sorting of Bacterial Lipoproteins to the Outer Membrane by the Lol System -- Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex -- Reconstitution of the SecY Translocon in Nanodiscs -- In Vitro Assays to Analyze Translocation of the Model Secretory Preprotein Alkaline Phosphatase -- Characterization of Interactions Between Proteins Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance Spectroscopy -- Analysis of Tat Targeting Function and Twin-Arginine Signal Peptide Activity in Escherichia coli -- Site-Specific Cross-Linking of In Vitro Synthesized E. coli Preproteins for Investigating Transmembrane Translocation Pathways -- Tracking the Secretion of Fluorescently Labeled Type III Effectors from Single Bacteria in Real Time -- Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-PAGE -- Using Hidden Markov Models to Discover New Protein Transport Machines -- Bioinformatics Predictions of Localization and Targeting -- The Chloroplast Protein Import Machinery: A Review -- Measurement of the Energetics of Protein Transport Across the Chloroplast Thylakoid Membrane -- In Vitro Dissection of Protein Translocation into the Mammalian Endoplasmic Reticulum -- In Vitro Reconstitution of the Selection, Ubiquitination, and Membrane Extraction of a Polytopic ERAD Substrate -- Studying the ArfGAP-Dependent Conformational Changes in SNAREs -- Studying Endoplasmic Reticulum Function In Vitro Using siRNA -- High-Quality Immunofluorescence of Cultured Cells -- Trapping Oxidative Folding Intermediates During Translocation to the Intermembrane Space of Mitochondria: In Vivo and In Vitro Studies -- Native Techniques for Analysis of Mitochondrial Protein Import.
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| 650 |
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0 |
|a Enzymology.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Proteomics.
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| 650 |
1 |
4 |
|a Enzymology.
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| 650 |
2 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Proteomics.
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| 700 |
1 |
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|a Economou, Anastassios.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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